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Fer‐1 inhibits Aβ 1–42 ‐induced PC12 cell injury, ROS production and iron accumulation. (A) Cell viability detected by MTT; (B) <t>LDH</t> content detected by <t>LDH</t> <t>assay</t> kit; (C) Intracellular ROS assay; (D) Fe 2+ concentration assay; (E, F) Levels of MDA and 4‐HNE were measured using commercial kits. Data are expressed as mean ± standard deviation. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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Fer‐1 inhibits Aβ 1–42 ‐induced PC12 cell injury, ROS production and iron accumulation. (A) Cell viability detected by MTT; (B) <t>LDH</t> content detected by <t>LDH</t> <t>assay</t> kit; (C) Intracellular ROS assay; (D) Fe 2+ concentration assay; (E, F) Levels of MDA and 4‐HNE were measured using commercial kits. Data are expressed as mean ± standard deviation. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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Fer‐1 inhibits Aβ 1–42 ‐induced PC12 cell injury, ROS production and iron accumulation. (A) Cell viability detected by MTT; (B) <t>LDH</t> content detected by <t>LDH</t> <t>assay</t> kit; (C) Intracellular ROS assay; (D) Fe 2+ concentration assay; (E, F) Levels of MDA and 4‐HNE were measured using commercial kits. Data are expressed as mean ± standard deviation. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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Fer‐1 inhibits Aβ 1–42 ‐induced PC12 cell injury, ROS production and iron accumulation. (A) Cell viability detected by MTT; (B) <t>LDH</t> content detected by <t>LDH</t> <t>assay</t> kit; (C) Intracellular ROS assay; (D) Fe 2+ concentration assay; (E, F) Levels of MDA and 4‐HNE were measured using commercial kits. Data are expressed as mean ± standard deviation. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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Fer‐1 inhibits Aβ 1–42 ‐induced PC12 cell injury, ROS production and iron accumulation. (A) Cell viability detected by MTT; (B) LDH content detected by LDH assay kit; (C) Intracellular ROS assay; (D) Fe 2+ concentration assay; (E, F) Levels of MDA and 4‐HNE were measured using commercial kits. Data are expressed as mean ± standard deviation. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: Journal of Cellular and Molecular Medicine

Article Title: TARDBP Mediates the MAP 3 K 11/ SLC 3 A 2/ GPX 4 Axis in Alzheimer's Disease Rats by Enhancing KRAS m RNA Stability

doi: 10.1111/jcmm.71181

Figure Lengend Snippet: Fer‐1 inhibits Aβ 1–42 ‐induced PC12 cell injury, ROS production and iron accumulation. (A) Cell viability detected by MTT; (B) LDH content detected by LDH assay kit; (C) Intracellular ROS assay; (D) Fe 2+ concentration assay; (E, F) Levels of MDA and 4‐HNE were measured using commercial kits. Data are expressed as mean ± standard deviation. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: LDH levels were measured using the LDH assay kit (CK12, Dojindo, Japan).

Techniques: Lactate Dehydrogenase Assay, ROS Assay, Concentration Assay, Standard Deviation

Up‐regulation of KRAS attenuates the ameliorative effect of down‐regulation of TARDBP on Aβ 1–42 ‐induced PC12 cells. (A) RT‐qPCR to verify successful transfection; (B) MTT to detect cell viability; (C) LDH assay kit to detect LDH content; (D) Intracellular ROS assay; (E) Fe 2+ concentration assay; (F, G) Levels of MDA and 4‐HNE were measured using commercial kits. Data are expressed as mean ± standard deviation. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: Journal of Cellular and Molecular Medicine

Article Title: TARDBP Mediates the MAP 3 K 11/ SLC 3 A 2/ GPX 4 Axis in Alzheimer's Disease Rats by Enhancing KRAS m RNA Stability

doi: 10.1111/jcmm.71181

Figure Lengend Snippet: Up‐regulation of KRAS attenuates the ameliorative effect of down‐regulation of TARDBP on Aβ 1–42 ‐induced PC12 cells. (A) RT‐qPCR to verify successful transfection; (B) MTT to detect cell viability; (C) LDH assay kit to detect LDH content; (D) Intracellular ROS assay; (E) Fe 2+ concentration assay; (F, G) Levels of MDA and 4‐HNE were measured using commercial kits. Data are expressed as mean ± standard deviation. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: LDH levels were measured using the LDH assay kit (CK12, Dojindo, Japan).

Techniques: Quantitative RT-PCR, Transfection, Lactate Dehydrogenase Assay, ROS Assay, Concentration Assay, Standard Deviation